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Nano toxicological studies of metallic oxide nanoparticles (zno, pb (no3)2)

A. Mohamed Sikkander

Metal oxide nanoparticles are widely used in the paint and coating industry as well as in cosmetics, but the knowledge of their possible interactions with the immune system is very limited as well as we are far from understanding how nanoparticles affect the human health. Most knowledge is derived from studies on cell lines that are reproducible and well standardized assays. However, it is unclear how well these results translate to primary human cells. Only a few studies have tested the effect of nanoparticles on human Peripheral Blood Mononuclear Cells (PBMC). The aim of the present study is to investigate if commercially available ZnO and Pb(NO3)2 nanoparticles may affect different human immune cells, nano-sized vesicles that have a role in cell to cell communication. Here found that PBMC reacted with a dose dependent manner, increase in cell death via cell viability to ZnO nanoparticles indicating different sensitivity in different immune cells. Here, PBMC were found to be strongly affected by increased doses of ZnO, with 54 ± 6% dead cells at 100μg/ml, whereas Pb(NO3)2 did not induce any significant cell death. Here concluded that subtoxic concentrations of ZnO nanoparticles do significantly alter PBMC characteristics, affect immune system and that evaluations of nanoparticles should be performed even at sub toxic concentrations on human blood cells when investigating potential effects on immune functions.