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Anticancer effects of Gardenia jasminoides in HepG2 human hepatoma cells

Shao-cheng Chen, Xin Zhao, Ruo-kun Yi, Jun Qian, Yan-hong Shi, Rui Wang

Background: Gardenia jasminoides is a natural plant, it has many biological activities. Its ability of cancer cell apoptosis needs to be researched.

Methods: In this study, the important biological activity materials were determined by high efficiency liquid chromatography. The in vitro anticancer effects were measured by MTT and RT-PCR assays in HepG2 human hepatoma cells.

Results: Gardenia jasminoides contained 1.03% genipin gentiobioside, 5.90% gardenoside, 1.26% crocin 1 and 0.17% crocin 2. Gardenia jasminoides had significantly strong HepG2 cells inhibitory effects, and highest effect 80.2% is showed in 400 μg/mL concentration of Gardenia jasminoides which is remarkable. the Gardenia jasminoides also have the ability to increase the mRNA expression of caspase-3, caspase-8, caspase-9, Bax, TRAIL, Fas, Fas/FasL, p53, p21, IκB-α and decrease the Bcl-2, BclxL, XIAP, cIAP-1, cIAP-2, survivin, NF-κB expression in HepG2 cells. The higher concentration of Gardenia jasminoides also showed the higher increasing or decreasing mRNA expression effects in HepG2 cells.

Conclusion: Gardenia jasminoides had a strong anticancer effect through its apoptosis inducing abilities in hepatic cancer; it could be used for the treatment as a medicine or health product in daily human life for good liver health.