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Gastrodin has in vitro anticancer effects in human glioma U118 cells

Yong Yang, Hanjiang Cui, Xian Wang

Gastrodin is a biologically active substance extracted from Gastrodia elata Bl. which is a traditional Chinese medicine. The aim of this study was to determine the in vitro anticancer effects in human glioma U118 cells. Two concentrations of gastrodin, (0.5, 1.0 and 2.0 mg/mL) both had the strong growth inhibitory effects in U118 cells determined by MTT assay. Using flow cytometry assay, high concentration of gastrodin (2.0 mg/mL) treated U118 cells contained the most apoptosis cells (43.2%) and the low concentration of gastrodin (1.0 and 0.5 mg/mL) treated U118 cells also had the more apoptosis cells (23.3% and 9.8%) than control cells (2.1%). By qPCR assay, the experiments results showed that gastrodin could raise caspase-3, caspase-8, caspase-9, Bax, p53, p21, IκB-α, Fas, FasL, TIMP-1, TIMP-2 and reduce Bcl-2, Bcl-xL, NF-κB, EGF, EGFR, VEGF, Fit-1, MMP-2, MMP-9 mRNA expressions. From these results, gastrodin could be used as a medicine for cancer treatment.