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Hypoxia promotes proliferation and invasion of osteosarcoma cell line SaOS-2 in vitro by activating Wnt/β-catenin signaling

Kaifu Wang, Zhenggang Bi

Objective: To investigate the role of Wnt/β-catenin signaling pathway on hypoxia induced proliferation and invasion in osteosarcoma cell line SaOS-2.

Methods: Cultured SaOS-2 cells were treated with hypoxia for 24 h. The proliferation rate was detected by CCK8; the mRNA and protein expression levels of β-catenin were detected by RT-PCR and Western blot; the effect of hypoxia on invasion ability of SaOS-2 cells was detected with Transwell method. Furthermore, β-catenin siRNA was used to investigate the role of β-catenin on hypoxia induced proliferation and invasion.

Results: The results demonstrated that hypoxia could promote SaOS-2 cells proliferation and invasion. The mRNA and protein level of β-catenin was upregulated by hypoxia treatment, whereas β-catenin siRNA could reverse these processes.

Conclusion: Hypoxia promotes osteosarcoma cell line SaOS-2 proliferation and invasion by activating Wnt/β-catenin signaling pathway.