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Impact of different concentrations of 1,25(OH)2D3 on expressions of AdipoR2, p38MAPK, LPL, and triglyceride in HepG2 cells

Yan Yang, Beibei Liu, Ling Gao, Qi Li, He Wang, Liandi Wang

The aims of this study were to observe the effects of different concentrations of 1, 25 dihydroxyvitamin D₃ (1,25(OH)₂D₃) on the content of Triglyceride (TG), as well as the gene and protein expressions of Adiponectin receptor 2 (AdipoR2), p38 Mitogen-Activated Protein Kinase (P38MAPK), and Lipoprotein Lipase (LPL) in TG’s metabolic pathway, in the HepG2 cells so as to provide theoretical basis for further studying the mechanism by which Vitamin D reduces TG. The HepG2 cells were treated with different concentrations of 1,25(OH)₂D₃ 0 ng/ml (CON), 12.5 ng/ml (L-dose), 25 ng/ml (M-dose), and 50 ng/ml (H-dose)) for 24 h, respectively, and then detected the gene and protein expressions of AdipoR2, p38MAPK, and LPL by Real-Time Polymerase Chain Reaction (RT-PCR) and Western blotting, as well as the content of TG by the enzyme method. Compared with Group CON, the gene and protein expressions of AdipoR2, p38MAPK, and LPL in Group L, M, and H-dose were all increased while the contents of TG were decreased (P<0.05). The levels of 1,25(OH)₂D₃ decreases the TG content in the HepG2 cells may be achieved by upregulating the expressions of AdipoR2, p38MAPK, and LPL.