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Isolation, molecular identification and genomic pattern of Mycobacterium bovis isolates collected from tuberculin-positive cattle in infected farms of Shiraz

Sachiko Isobe

Fifty pathological samples from tuberculin-positive cattle collected from two slaughterhouses at Shiraz were subjected to bacterial culture on glycerinated and pyruvated Lowenstein-Jensen media. Genomic material from culture-positive slopes was extracted and used in PCR-16S rRNA, PCR-IS6110, and PCR-RD typing experiments. All the M. bovis isolates were further genotyped using pvu II-digested PGRS-RFLP strategy