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Purification of (S)-2-methyl-1,4,5, 6-tetrahydropyrimidine-4 carboxylic acid from Halophiles

Chen Rui-Feng, Lv Li-Huo, Yao Su, Zhu Li-Jun, Li Bin, Qian Jun-Qing

Aim: This paper is focused on the techniques of fermentation, separation and purification of (S)-2- methyl-1,4,5, 6-tetrahydropyrimidine-4 carboxylic acid generated by Halomonas elogate 1A01717.

Methods: This study take Halomonas elogate 1A01717 to ferment to produce a total of 97.05 Kg fermented liquid, and carry out purification process including ceramic membrane microfiltration, electrodialysis desalination, diatomaceous earth filter, upper column, crude extraction and refining.

Results: Three experimental processes are stable, getting total Ectoine 634.7 g, total yield: 45.17%. The yield of 6.04 g/L is calculated according to material coefficient 70%, 35 L of 50 L fermentation canned. Finally, the products getting in the three tests quality conforms to the standard.

Conclusion: This process can be used for a large-scale production and purification of ectoine after further optimization and adjustment.