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Simultaneous determination of six active constituents of Inula flowers dispensing granule by HPLC-DAD

Ping Zhang, Min Zheng, Yimin Cai, Peijun Cai

Objective: To develop an HPLC-DAD method for simultaneous determination of chlorogenic acid, caffeic acid, 1, 5-dicaffeoylquinic acid, rutin, spinacetin and quercetin in Inula flower dispensing granule.

Methods: Chromatographic separation was performed on a thermo hypersil BDS C18 column (4.6 mm × 250 mm, 3 μm) with a 0.5% citric acid-acetonitrile gradient at the flow rate of 1 ml/min. The column temperature was maintained at 35°C, and detection wavelength was set at 360 nm.

Results: Chlorogenic acid, caffeic acid, 1, 5-dicaffeoylquinic acid, rutin, spinacetin and quercetin were linear in the ranges of 0.101-2.020 μg (r=0.9998), 0.052-1.040 μg (r=0.9997), 0.210-4.200 μg (r=0.9997), 0.142-2.840 μg (r=0.9998), 0.045-0.900 μg (r=0.9996) and 0.050-1.000 (r=0.9997), respectively. The average recoveries were 98.37%, 98.57%, 99.11%, 98.93%, 98.81% and 99.26%, respectively, and the RSDs were 0.82%, 0.91%, 0.86%, 1.19%, 0.93% and 0.86%, respectively.

Conclusion: The HPLC-DAD method was accurate, convenient and reproducible for quality control of the multi-component in Inula flower dispensing granule.