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The influences of icariin on SDF-1 and CXCR-4 of rats with acute ischemic stroke and study on its protection function for nerve

Wei Rong, Yong Yan, Chun Yan Li, Xin Rui Li, Li Yang

Objective: To explore influences of icariin on Stromal-Derived Factor-1 (SDF-1) and CXC Chemokine Receptor 4 (CXCR-4) of rats with acute ischemic stroke and study on its protection function for nerve.

Methods: Healthy SD rats were selected, they were divided into the sham operation group (N group), model control group (M group), high and low icariin dosage group (T1 and T2), rats were given normal dieting for 24 h after model build, and intragastric administration, of which, N group and M group were given intragastric NS, rats in low dosage and high dosage group were given 30 mg/kg and 60 mg/kg icariin separately, once one day, last about 14 d, cutting off neck and sudden death. 3 rats were selected from M group, N group, T2 and T1 and given NSS. Rats in each group were given evaluation in 2 h, 1, 7 and 14 d’s after surgery and given PCR measurement according to evaluation results.

Results: Neurological impairment in M group after surgery reached to 1 d, then decreased gradually, neurological score of T1 compared with model control group, there were no statistical differences (P>0.05), compared with T2 group, there were statistical differences (P<0.05). According to results of this test, from detection results of gene in M and T2 group we can know, SDF-1 and CXCR-4 gene in T2 group were higher than M group, but there were no statistical differences (P<0.05) in 7 d. Gene in T2 two groups were higher than control group through comparison between SDF-1 and CXCR-4 gene level of two groups in 14 d, the differences of SDF-1 and CXCR-4 had significant (P<0.05), the comparison differences of SDF-1 gene level were extremely significant (P<0.01).

Conclusion: Icariin can promote neurological function recovery of rats after ischemia, its mechanism may have relations with icariin up-regulating SDF-1 and CXCR4.