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MicroRNAs as molecular markers for screening of colon cancer

Farid E. Ahmed, Nancy C. Ahmed, Mostafa Gouda, Chris Bonnerup

The importance of melt curve analysis (MCA) is demonstrated by a foodomic study that we have carried out to facilitate interpretation of mild expression data, by measuring the magnitude of the expression of key micro(mi)RNA molecules in stool of healthy human adults that have been used as molecular markers, following the intake of Pomegranate juice (PGJ), functional fermented sobya (FS), rich in probiotic lactobacilli, or their combination. Total small RNA was isolated from stool of 25 volunteers, 20 to 34 years old, before and following a three-week dietary intervention trial. Expression of 88 miRNA genes was evaluated using Qiagen's 96 well plate RT2 miRNA qPCR arrays.